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It also necessitates multifactorial strategies to minimise the risk of possible acute and long-term complications such as neuropathy, retinopathy, and nephropathy and cardiovascular morbidities which are increased two-to-threefold in these patients and are subsequently a major cause of death

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Briefly, a 24-well culture plate was coated with 200 l (9.1 mg/ml)/well Matrigel prepared from Engelbreth-Holm-Swarm mouse tumors (Corning, MA, USA) and allowed to stand for 30 min at 37 C to form a gel layer