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Description

Disruptions in iron metabolism leads to the accumulation of intracellular free iron, which catalyzes the generation of reactive oxygen species (ROS) through the Fenton reaction

The resulting systems were prepared for simulations using in-house scripts based on HTMD2.3.2 45 and VMD1.9.4 frameworks

Mutants selected with pyrimethamine (input 2) were then used to co-infect mice with a WT parasite line that constitutively expressed Cas9 under an hsp70 promoter ( cas9 )