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Three phases of detoxification:[ref] Phase I (bioactivation transformation) involves breaking apart large toxins and converting them into polar molecules

f , Immunoblot analysis of indicated proteins from SLC33A1 -knockout HEK293T cells expressing a vector control or SLC33A1 cDNA

This second step requires additional nutrients such as Vitamins B5, folic acid, and B12, Vitamin C, magnesium, amino acids such as methionine, cysteine, glutamine, choline, taurine, and glycine