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Wait a few seconds after full injection before withdrawing the needle at the same angle it was inserted

It contributes to cell membrane integrity and acts as a precursor for acetylcholine, a neurotransmitter important in memory, attention, and learning

Echocardiographic assessments were performed with sedation using 50 mg/kg of intraperitoneal ketamine and 5 mg/kg of xylazine, ensuring stable positioning