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Description

Lysosomal calcium signalling regulates autophagy through calcineurin and TFEB

Xia N, Daiber A, Forstermann U, Li H

Once in the cytosol, Glu and Cys are combined into the dipeptide -glutamylcysteine (-GC) by the enzymatic activity of glutamatecysteine ligase (GCL), which requires ATP and Mg 2+ as co-substrates (Figure 2A) [15,16,17]

GPX4 specific activity was carried out in a coupled assay, by monitoring the consumption of NADPH in the presence of glutathione reductase upon addition of phosphatidyl choline hydroperoxide (Roveri et al., 1994)