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Disclosures: Davide Panzeri: Nothing to Disclose, Luca Di Tommaso: Nothing to Disclose, Donato Inverso: Nothing to Disclose, Laura Sironi: Nothing to Disclose 2557 CAS9 3-BASE & PAM OFFSET (C3PO) DETECTION INCREASES DELETION EFFICIENCY TO HELP STUDY PNPLA3 MUTANT KNOCKOUTS IN HUH7 CELLS Chinmay Raut 1 Rawdat Hussain 2 Akram Irshaid 2 Prabhu Ponnandy 2 Elizabeth Speliotes 3 , 1 University of Michgan Ann Arbor, 2 University of Michigan Ann Arbor, 3 University of Michigan Medical Center Background: PNPLA3 I148M is associated with metabolic dysfunction-associated steatotic liver disease (MASLD) and metabolic dysfunction-associated steatohepatitis (MASH)

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This could be the reason for mitigated misdirection of regenerating tibial motor axons and ameliorated chronic flexion contracture observed in the present study

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