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Tissue GST activity assay: Enzymatic reaction: Homogenate supernatant (ml) Mix sufficiently, centrifugate 3500~4000rpm for 10 minutes, take supernatant for chromogenic reaction Chromogenic reaction: Calculation: Unit definition: 1mg tissue protein reacts at 37 for 1 minute, subtract non-enzymatic reaction, 1mmol/L GSH concentration reducing in reaction system is considered as 1 enzyme activity unit (U)

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