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Description

After 5 min at room temperature, the solution was subjected to centrifugation at 3000 rpm for 15 min

Funding This work was funded by grants from the National Natural Science Foundation of China (82303590), the Youth Innovation Team Development Program of Shandong Province (2023KJ227), the China Postdoctoral Science Foundation (2025T180977 and 2023M741867), the Postdoctoral Innovation Program of Shandong Province (SDCX-ZG-202400078), the Youth Science and Technology Innovation Project of Shandong Provincial Maternal and Child Health Care (SFYZXJJ-2024030), Shandong Traditional Chinese Medicine Technology Project (2020M108), Qingdao Traditional Chinese Medicine Research Plan Key Project (2020-zyz002), Qingdao Science and Technology Plan Project (2019-WJZD052), and the Qingdao Postdoctoral Science Foundation (QDBSH20230202076)

We found that the oxidative stress response pathways SKN-1, SEK-1, and HLH-30 were essential for lifespan extension on mutant diets and under iron-depleted conditions