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Following this, coverslips were blocked with 2% bovine serum albumin in PBS for 1 h at room temperature and subjected to immunofluorescence staining using an NFE2L2 antibody, followed by Alexa Fluor 488-conjugated IgG

Additional reference Sir Frederick Gowland Hopkins Sir Frederick Gowland Hopkins was awarded the Nobel Prize in Physiology or Medicine in 1929, with Christiaan Eijkman, for the discovery of vitamins

80 This treatment was also found to be safe with minimal side effects including indigestion and marginal bradycardia (propranolol effect)