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In our model we use a V max of 1100 M/hr, a K m of 3000 M, and a Hill coefficient of 3

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2 h post-infection, cells were washed with cold PBS and lysed with TBS buffer containing 150 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 1% Triton X-100, following with 10% sonication for 10 s

For the other immune molecules, the expression level of those genes reached the highest level in stage C or stage D and the lowest level in E stage or AB stage, such as metallothionein , metallothionein 2 , macrophage migration inhibitory factor , macrophage migration inhibitory factor MIF1 , hemocyanin subunit 1 , hemocyanin subunit 2 , and hemocyanin subunit 5