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Yang C, Zhu Y, Tian Z, Zhang C, Han X, Jiang S, et al

Some people lose weight quickly at first, while others see a steadier pace and both patterns can still lead to strong long-term results

The membranes were blocked with 5% BSA and incubated with primary antibodies at 4 C for 12 h, followed by secondary antibodies (HRP-conjugated Goat Anti-Mouse IgG, Proteintech, SA00001-1, 1:20000) for 1 h at room temperature