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111 3-MST uses 3-MP, derived from cysteine through the action of CAT, as an activating substrate to generate a persulphide at the catalytic Cys248 residue

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Analysis of changes in cerebral glutamine, choline and inositols

For example, use standard molecular biological technique that total length or Partial cDNA Sequence are cloned into recombinant expression carrier and said carrier transfection is got into cell.Can be for example through using polymerase chain reaction (PCR) to increase or obtaining cDNA through screening suitable cDNA library.The nucleotide sequence of cDNA can be used for designing the PCR primer that allows to utilize Standard PC R method amplification cDNA or is used to design the hybridization probe that can be used for using standard hybrid method screening cDNA library.After separating or increasing cDNA, said dna fragmentation is introduced suitable expression