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Electron microscopy Cells were washed with PBS and centrifuged at 2000 rpm for 5 min

Being polyphenols emitting at 280 and 320 nm extracted by PBS together with total soluble proteins (anthocyanins are not efficiently extracted, Supplementary Figure 4), the contribution of these classes of molecules may be hypothesized in stabilizing proteins, as proofs of the interaction of anthocyanins with proteins seem to suggest (Sui et al., 2018)

The caliper was used to measure the size of the tumor, with tumor volume calculated using the formula V = L W2 /2, where L and W represented the length and width of the tumor