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The total cell lysate was cleared by centrifugation and supplemented with 25 L per liter culture OD 600 of BioLock avidin (IBA Lifesciences, Gttingen, Germany) to mask D-biotin from the culture medium and/or endogenous biotinylated proteins from the host cell, followed by sterile-filtration

Multiple sites on SARS-CoV-2 spike protein are susceptible to proteolysis by cathepsins B, K, L, S, and V

It is naturally produced by the liver and comprises the amino acids cysteine, glycine, and glutamine

Substrate (e.g., glucose) consumption and oxygen consumption continue, and will actually increase, because the protons are not being pushed out against a gradient and just flow right back in without doing any work to make ATP