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The anterior segment, the lens and the retina were removed, and the RPE/choroid tissue (posterior eye) was treated with 0.5% Triton X100 in 5% blocking buffer (with blocking serum in which the secondary antibody was raised) and subsequently used for immunolabeling with antiCD31 antibodies to detect blood vessels, Alexa488conjugated phalloidin, and antigalactosidase antibody to confirm lacZ expression in the RPE as a genotype control

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