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And then, the cells were sonicated two times with 30 bursts of 1 s each (Branson Sonifier 250, output control 3, duty cycle 30%)

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Oral administration of poly--glutamate, a natural product of a few Gram-positive bacteria, including Staphylococcus and Bacillus species, ameliorates AD-like dermatitis in Nc/Nga mice by suppressing the Th2-biased immune response and production of IL-17A, which may indicate it to be a desirable prebiotic for treating overactive Th17 cells involved in psoriasis (Lee et al., 2014)