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Chronic ROS exposure by long-term ethanol feeding induces mild steatosis and slightly elevation of serum ALT, with little or no liver inflammation

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In particular, NAD was found to bind to P2Y1 receptor and act as an inhibitory neurotransmitter at neuromuscular junctions in visceral smooth muscles (12)

Below are the specific protocols discussed on the Huberman Lab Podcast

TMT liquid chromatography-mass-spectrometry (LC-MS) analysis Samples were analyzed by liquid chromatography-tandem mass spectrometry using an Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Scientific) coupled to an UltiMate 3000 Series Rapid Separation LC system and autosampler (Thermo Scientific Dionex)