elution reduced glutathione for gst-egfp fusion protein Transglutaminase 2 crosslinks the S-transferase tag, impeding protein–protein interactions of the fused GFP expression in E. coli.
GFP expression in E. coli. (A) Purification workflow of GST fusion Download Scientific Diagram In vitro GST pull down assay. (A) A GST fusion protein (red) is Download Scientific Diagram Deciphering the mechanism of glutaredoxin catalyzed roGFP2 redox sensing reveals a ternary complex with glutathione for protein disulfide reduction Nature Communications GST Tag Definition & Data
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