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The chromatography resin utilizes the interactions between Ni2+ and side chains of some amino acids (mainly histidine) in the protein to separate and purify proteins with and without these amino acids and with high or low amounts of these amino acids

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2 Ferroptosis 2.1 Overview Ferroptosis is defined as a type of programmed cell death that is dependent on iron and distinct from apoptosis, a concept first proposed by Dixon and his team in 2012 (Park et al., 2023)

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