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We observed that the intracellular bacterial pathogen Listeria monocytogenes is highly resistant to the bactericidal effects of 4-HNE and that several specific transcriptional responses are induced in response to toxic 4-HNE exposure, including two enzymes, rha1 and rha2 , whose loss sensitizes L

The muscle here is the vastus lateralis

Jiang H, Galtes D, Wang J, Rockman HA

Cells were then washed and stained for intracellular markers 30 min at 4C and acquired by CytoFLEX LX Flow Cytometer (Beckman Coulter, RRID: SCR_025067)