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Innovative sarcoma therapy using multifaceted nano-PROTAC-induced EZH2 degradation and immunity enhancement

There are four different methods namely dose by factor, similar drug, pharmacokinetically guided, and comparative approaches are described in literature to assess the initial dose.[1] The dose by factor method is an empirical approach and use the no observed adverse effect levels (NOAEL) of drug from preclinical toxicological studies to estimate human equivalent dose (HED).[2] Here, the dose selection is based on minimum risk of toxicity, instead of choosing one with minimum pharmacologic activity in humans

While the samples treated with Zn (1 g L 1 ) x GSH (0.2 g L 1 ) give GTS percentage 75%, this may be effect on gene expression of genetic material and give the greatest number of 16 and 17 bands, respectively

Quercetin was used as the standard to generate a calibration curve