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An actin gene was included as a reference gene (internal control)

this indicates that there is some flexibility in nutrient usage, which is crucial to support cellular fitness (Fig

Redox-state analysis DF-1 cells were lysed by freezethaw method, and reduced/oxidized glutathione (GSH/GSSG) measurement was performed using a GSH and GSSG Assay Kit (Beyotime) according to the manufacturers protocol