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Carbas, B., Machado, N., Pathania, S., Brites, C., Rosa, E

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Twice-daily dosing: Best for acute injuries Maintains more consistent peptide levels Often used for the first 12 weeks Morning and evening Total daily dose can be split between injections Once-daily dosing: Suitable for chronic conditions More convenient for long-term protocols Often used after the early healing phase Morning dosing is often preferred Full daily dose in a single injection Timing factors: Can be administered any time of day Consistent timing improves adherence No specific relationship to meals needed No specific timing relative to exercise needed Many researchers prefer morning dosing for convenience Localized vs

The sham surgery group (referred to as normal cartilage) was sacrificed at 12 weeks, and the only-defect groups with PBS injection (referred to as only-defect) were sacrificed at 4, 8, and 12 weeks when the injection groups were sacrificed