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Optimization included testing the inclusion of gp42 alone or with gp350, the effect of the order of the two glycoproteins gH and gL in tandem expression constructs, the timing and dosing of repRNA delivery, the effect of soluble versus membrane-anchored antigen, and the delivery of gH/gL fused to different multimeric constructs encoded by repRNA

Our peptide reconstitution guide covers the mechanics of how vial volume, peptide mass, and diluent volume determine final concentration, and why the diluent has to physically fit the vial

In this study, we submitted mice to monthly episodes of APAP intoxication starting as a young adult (90-days-old) until an elderly age of 420 days