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The GM of children is characterized by relatively higher abundances of microbes with genes that function to support human development, including vitamin biosynthesis and polysaccharide and xenobiotic metabolism (84)

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For the final steps of mutant construction and confirmation, recombinant colonies were selected by plating on 7H10-ADC with the appropriate antibiotic/supplement and confirmed by PCR using specific primers (Supplementary Table 2 and Fig

Ivanov et al., 2005