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The loss of wild-type p53 (wt-p53) activity and acquisition of oncogenic gain-of-function, secondary to aberrant accumulation of mutant-p53 (mut-p53) protein, frequently results in aggressive tumour phenotypes and poor survival 1

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Cell Mol Life Sci 69: 23272343, 2012 [DOI] [PMC free article] [PubMed] [Google Scholar] 2.Ata H, Rawat DK, Lincoln T, Gupte SA

Cells were resuspended in 250 l relevant culture medium supplemented with rho-kinase inhibitor Y-27632 (overnight) and optionally long term with 5 nM scTS2/16