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If there is a lack of cobalamin in the body, methylmalonyl CoA accumulates, which presumably leads to the neurological manifestations of B12 deficiency.[4][7][9][12] The replenishment with parenteral cyanocobalamin causes a rapid and complete improvement of megaloblastic anemia and gastrointestinal symptoms caused by vitamin B12 deficiency

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All mice experiments were conducted under protocols that had been previously reviewed and approved by the University of Calgary's Veterinary Sciences Animal Care Committee (VSACC) with the reference number AC17-0220.Various groups of mice (n=14) were intraperitoneally inoculated