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To validate the in vitro findings of ICD induction and STAT3 suppression, HMGB1 and CRT expression was examined via immunohistochemistry, whereas STAT3 and phosphorylated STAT3 levels were analyzed via western blotting

The cells were then treated with a serum-free medium containing MSU (4 g/mL), Mn + (1.5 g/mL), or a MnUA combination

Additionally, the neuroprotective scorpion venom heat-resistant peptide (SVHRP) exhibits neuroprotective effects by reducing neurological deficit scores, inhibiting edema formation, decreasing infarct volume and neuronal loss, and protecting primary neurons against oxygen-glucose deprivation/reoxygenation (OGD/R)-induced injury (Wang et al., 2020)