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MC4R is found in the brain (namely in the medial preoptic area) and is crucial for regulating sexual behavior, energy, and appetite

The enzymes using ATP as substrate in astrocytes belong to a broad range of enzyme classes (EC), including EC2 (transferases i.e., hexokinase), EC3 (hydrolases i.e., NTPase, nucleoside triphosphatases), EC4 (lyases i.e., adenylyl cyclases), EC5 (isomerases i.e., dynein ATPase), EC6 (ligases i.e., glutamine synthetase) and EC7 (translocases i.e., Na + -K + -ATPase)

Prior analyses, the internal standard verapamil- 13 C3 hydrochloride (Merck) was dissolved in methanol and spiked into samples to a final concentration of 200 nM