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Ni 2+ -loaded chelating Sepharose was used for the affinity purification of the target protein (Figure 2B, lanes 13 right )

Melatonin prevents against ethanol-induced liver injury by mitigating ferroptosis via targeting brain and muscle ARNT-like 1 in mice liver and HepG2 cells

The targeting sequence will be sufficiently near the 5 end of the polynucleotide sequence so the promoter will be operably linked to the endogenous sequence upon homologous recombination