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tt SI and conSI were solubilized in 1%(w/v) DDM, 0.2%(w/v) CHS, 500 mM NaCl, 1 mM PMSF, 1 mM TCEP, 0.1 protease inhibitor cocktail, 2 mM imidazole, 50 mM Tris-HCl pH 8.0

Therefore, the absence of an association between selenium status and NWCS is unsurprising

Based on this, we generated the relative binding abundance percentage of the 12 cell types measured by snRNA-seq within the 15 clusters of the spatial transcriptome