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Additionally, irisin downregulated the downstream target protein hypoxia-inducible factor-1 (HIF-1) and upregulated peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1)

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[5] Upon binding, GHRP-6 may induce a conformational change in GHS-R1a, potentially activating the phospholipase C (PLC) pathway, which may lead to the production of inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), two secondary messengers involved in cellular signaling