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Am J Gastroenterol 97:14381444 Lamarque D, Whittle BJ (2001) Increase in gastric intramucosal hydrogen ion concentration following endotoxin challenge in the rat and the actions of nitric oxide synthase inhibitors

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Proteins were diluted to 25 g in Pierce RIPA Buffer lysis reagent with 1X Laemmli Sample Buffer (Bio-Rad) containing 10% -mercaptoethanol and incubated for 10 min at 70 C