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The splicing ratio was calculated separately for 5SS and 3SS by dividing the number of spliced reads by the total number of spliced and unspliced reads crossing the respective SS

Interestingly, PEPT2 may also be expressed differently during development and aging, according to a thorough review by Wang and collaborators [129]

Briefly, harvested cells were suspended in a hypertonic solution of 30 mM Tris, 20% w/v sucrose, 1 mM ETDA, pH 8 (25 mL) and incubated for 30 min at 4 C

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