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Total RNA was extracted from dermal fibroblasts using the RNeasy method (Qiagen, Venlo, The Netherlands), followed by DNase digestion (RNase-Free DNase set, Qiagen) according to the manufacturers protocol

No patient who was node-negative and carrying the double null-GSTT1-GSTM1 genotypes had poor response to chemotherapy

Do NOT layer in the same routine with retinol, high-strength vitamin C, or AHA/BHAalternate nights instead

Therefore, we investigated the role of B12 deficiency on lipid metabolism and inflammatory mediators in both in vitro and ex vivo models including human pre-adipocytes, primary adipocytes, mature human white AT (WAT), and its association with metabolic risk