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the following morning (Malinowski et al

Severe acute respiratory syndrome coronavirus ORF3a protein activates the NLRP3 inflammasome by promoting TRAF3-dependent ubiquitination of ASC

Dissected pieces were permeabilized and preincubated in a solution containing 5% normal goat serum and 0.5% Triton X-100 in phosphate-buffered saline (PBS) at 24 C for 1 h