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Gong, Zhicheng

Metabolic control of autoimmunity and tissue inflammation in rheumatoid arthritis

19 BianS.JiangY

Cell fractionation Cells were resuspended in fractionation buffer (250 mM sucrose, 20 mM Hepes, 10 mM KCl, 1.5 mM MgCl 2 , 1 mM EGTA, 1 mM EDTA, 1X protease inhibitor cocktail) and then sonicated (4 15 s, 50% amplitude)