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We based this conclusion on the observation that there are two subregions of SD1 and SD2 that are much similar to each other as compared to rest of the sequence in these duplicated segments (Fig

SUMOylation analysis in human brain tissue 20 mg of frozen human brain tissue was homogenized in 300 L of RIPA buffer supplemented with protease inhibitor, phosphatase inhibitor, and 50 mM N-Ethylmalamide and vortexed every 5 min for 20 min while incubating on ice

Additionally, sterile water is hypotonic and lacks the stabilizing agents necessary for keeping peptides viable for more than 24 hours