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Cell lysates and NMR experiments in lysates U-251 MG human brain glioblastoma cells (CLS Cell Line Service, Eppelheim, Germany) were cultured in Dulbeccos Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum and 0.1% PenicillinStreptomycin (Biowest, Nuaill, France)

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The ratio of the fluorescence emission intensity upon excitation at 405 nm and 488 nm reflects changes in the dithiol disulfide state of the probe and, in combination with fully oxidized and reduced controls, allows the determination of the degree of the probe oxidation independent of probe concentration (Degrossoli et al., 2018

Therefore, achieving a balance between toxicity and transfection efficiency is a major challenge in the design of cationic polymers