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2.5 Bone marrowderived macrophages Bone marrow cells from C57BL/6 and MyD88 -/- mice were obtained by washing the femurs and tibias with cold PBS and the stem cells were cultured in petri dishes in the presence of DMEM (Gibco) with 10% FBS, 1% HEPES, penicillin G sodium (100 U/ml), streptomycin sulfate (100 g/ml), and 20% L929 cell conditioned medium (LCCM) containing macrophage colony-stimulating factor (M-CSF)