selenium supplementation glutathione peroxidase The role of metabolism and selenoproteins in cartilage homeostasis and arthropathies A Comprehensive Review of Selenium

doi: 10.1002/smll.202308397 64 ZhaoCWangCShanWWangZChenXDengH

Ethics declarations Conflict of Interests The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest

doi: 10.3389/fcimb.2024.1339750 56 FanY.ChenX.ShanT.WangN.HanQ.RenB.et al

For cDNA synthesis, 3 g of total RNA was reverse transcribed in a 20L reaction mixture containing 1 L of random oligonucleotide primers, 1 L of 10 mM dNTPs, 4 L of 5X RT buffer, 1 L of 100 mM DTT, 1 L of RNAseOUT TM (40 U/L), 1L of SuperScriptR TM IV reverse transcriptase (200 U/L) (Invitrogen, Waltham, USA), and RNase-free water