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Nile Red (#19123, Sigma-Aldrich, Shanghai, China) solution at a concentration of 10 M was added and incubated for 10 min

5%EGCG cream is the optimal concentration for the treatment of vitiligo (Zhu et al., 2014)

Study groups were evaluated by standard physical examination, chest X-ray, baseline electrocardiogram, two-dimensional echocardiography, and routine clinical laboratory tests, including liver and kidney function tests and 24-hour urinary protein measurements