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RSL3 does not inhibit cystine uptake and yet triggers an otherwise similar iron and ROS-dependent ferroptototic death program

This is why responsible protocol design usually starts with four variables working together: Mechanism: Match the amount used to the peptides signaling role, rather than assuming more compound creates a better effect

The formazan product was solubilized with sodium dodecylsulfate (SDS, 10%, m v 1 in 20 mM HCl), and the absorbance was measured at 595 nm relative to the absorbance of vehicle (DMSO)-treated control cells using a multiwell scanning spectrophotometer (Victor 3 plate reader, PerkinElmer, Rodgau-Juegesheim, Germany)