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Typically, cells were seeded in 100 L RPMI phenol red free media per well in duplicates on a black 96-well plate with clear bottom (Thermo Scientific)

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In practical terms, it accelerates healing in gut lining, tendons, ligaments, muscle, and joint tissue by restarting a repair process that has stalled or by supporting tissue that is under chronic inflammatory stress

When Zhiyue Lu of the University of North Carolina read about the Mpemba effect in middle school, he snuck into an oil refinery in the Shandong province of China where his mother worked and used precision lab equipment to measure temperature as a function of time in a sample of water (he ended up supercooling the water without it freezing)