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Using a lesser diluent, like simple sterile water, is possible for single-use applications, but it offers no protection against contamination once the vial's septum has been punctured

For example, the triumvirate of gasotransmitters (CO, H 2 S, and NO) share similar effects in several aspects, act on the same transduction pathways, and show crosstalk among each other 58

The structure is what gives the peptide its specific function, and aggressive handling can physically damage that structurea process called denaturation