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For cell cycle analysis of cells embedded in soft agar, cells (10 5 ) were suspended in 1 mL of 0.33% noble agar, seeded in the bottom of 15-mL polypropylene tubes, cooled for 5 min on ice, and then kept at 37 C in a humidified incubator in the presence of 5% CO 2 for 24 h72 h

Each parameter was cross-referenced with experimental data to ensure that the numerical simulations aligned with real-world biological phenomena

Building on this foundation, our purpose is to drive change to defeat serious chronic diseases from diabetes and obesity to rare blood and endocrine disorders by pioneering scientific breakthroughs, expanding access to medicines, and working to prevent and ultimately cure disease

Kwan, V., Unda, B