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EC 2.3.2.13) is an acyl transferase that forms both intra- and inter-molecular isopeptide bonds in and between proteins

[DOI] [PMC free article] [PubMed] [Google Scholar] 4.Harper JM, Leathers CW, Austad SN

Each Cy5-labelled DNA was added to hybridization buffer (20 l DIG Easy Hybridization Buffer, 1 l 10 mg/ml Yeast tRNA and 1 l 10 mg/ml Salmon Sperm DNA) and hybridized for 18 hours at 50 C followed by washing 3 1 SSC/0.1% SDS at 42 C and final wash in 0.1 SSC at RT

Western blot analysis revealed elevated SHP-1 expression and reduced phosphorylated STAT3 (p-STAT3) expression in glioblastoma cells treated with AC compared to controls