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Materials and Methods hPSC Culture The hPSC lines utilized in this study were as follows: the human embryonic stem cell lines H1 (WiCell) and 207 4 and the previously described human induced pluripotent stem cell (hiPSC) lines AG27 (reprogrammed using retrovirus (Vectalys) from AG05836B fibroblasts, obtained from Coriell Cell Repositories) 5,6,7,8

Results Effect of different treatments on water intake, urine output, and renal toxicity markers Water intake and urine output were checked one week after DOX injection (Table 1)