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Description

Characterization of inflammatory response in hepatorenal syndrome: Relationship with kidney outcome and survival

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Cells were incubated with ACK lysis buffer (Quality Biological) for 5 min at room-temperature, washed twice with complete DMEM, treated with HBSS (Corning) containing 0.1 mg/mL DNAse I (Sigma-Aldrich, D4513) for 5 min, and then strained through a 20um pre-separation filter (Miltenyi)