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Sometimes DNase I and/or EDTA are included in the final cell suspension medium to reduce any cell aggregation

While it has been previously suggested that the ancestor of the Onygenales very likely was heterothallic 39 , the placement of multiple lineages of homothallic species in this group raises the possibility of a homothallic ancestor, with more recent transitions in pathogenic species to be heterothallic and rearrangements of the mating locus in others such as P

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Avoid repeated freeze-thaw cycles, as this can degrade peptides